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The response of phytoplankton to variations in the light regime was studied during the VULCAN and ACDA cruises in the Antarctic. Unenriched batch cultures of 12–19 days' duration reached chl concentrations of 10–50 μg−1 and exhibited exponential growth rates, with the maximal rate being 0.41 doubl, day−1. Ice edge algae exhibited maximum growth rates at photon flux densities (PFD) of 30–100 μE m−2S−1 and the growth rate was reduced by about 30% at 500–1000 μE m−2S−1 The chl/C ratio ranged between 0.004 and 0.018, with the lowest ratios at PFDs above 500 μE m−2S−1 chl/C ratios were also below maximum at PFDs below 40–50 μE m−2S−1 The C:N:P ratios were close to the Redfield ratios; the Si/C ratio averaged 0.16 (atoms), and the ATP/C ratio averaged from 0.0024 to 0.0050 in different culture senes. When thawed after having been frozen for 10 days, shade-adapted cultures were in a much better condition than sun-adapted ones. P versus I data showed that the maximum assimilation number varied from 0.75 to 4.4 μg C (μg chl)−1h−1. It varied inversely with the chl/C ratio; therefore the maximum carbon turnover rate varied little between samples (0.024/0.035 h−1). Low biomass communities exhibited relatively high values for α (the initial slope of P versus I curves), low values for 1sat (160–330 μE m−2S−1), and they were susceptible to photoinhibition. In contrast, communities dominated by Odontella weissflogii exhibited low values for α, a high value for Isat (560 μE m−2S−1 and they tolerated high PFDs. The photo-adaptational status of the phytoplankton in natural water samples is discussed relative to the profile of water column stability and mixing processes.

A mathematical model describing the development of phytoplankton blooms as a function of the depth of the wind-mixed layer, spectral distribution of light, passage of atmospheric low-pressure systems, size of the initial phytoplankton stock and loss rates is presented. Model runs represent shade-adapted, large-celled, bloom-forming diatoms. Periodic deep mixing caused by strong winds may severely retard the development of blooms and frequently abort them before macronutrients are completely exhausted. Moderate depths of mixing (40-50 m) in combination with a moderately large total loss rate (about 0.01 3 h-1) can prevent blooms from developing during the brightest time of the year. Complete exhaustion of macronutrients in the upper waters is likely only if the wind-mixed layer is less than 10 m deep, i.e. in very sheltered waters, and also in the marginal ice zone when ice is melting. We do not exclude the possibility of control of phytoplankton biomass by iron in ice-free, deep-sea parts of the Antarctic Ocean, but the implied enhancement of export production through addition of iron might be restricted because of limitation by light, i.e. vertical mixing.

Fifteen oceanographic stations were occupied in the vicinity of Anvers Island, Antarctica, in January of 1985 and 1987. All stations showed high phytoplankton biomass (4.0 to 30 μg chl-a/liter) which was either uniformly distributed in the upper mixed layer or showed a pronounced sub-surface maximum at 4–5 m depth. As phosphate was less than 0.02 μm and nitrate about 2.0 μm in surface waters, it appears that nutrient limitation of phytoplankton growth may be of importance during such blooms. This view is supported by chemical measurements of the particulate material which showed high chl-a/ATP ratios (about 7.7), as well as high POC/ATP ratios (about 700). Microscopical analysis revealed a dominance of large-celled diatoms and the near absence of heterotrophic protozoans. Size fractionation studies showed that the nanoplankton accounted for only 28% of the total phytoplankton biomass. When phytoplankton biomass reaches the levels found at these stations, it appears that the cells are light-limited and hence dark-adapted, which results in the high chl-a/ATP ratios and the low assimilation values (0.49–1.64) obtained in our studies. Under such conditions greater than 50% of the total phytoplankton biomass is found below the 1% light level.

Microscopical examination of near-surface eucaryotic microbial populations in circumcontinental waters of Antarctica indicated that nanoplankton (<20 μm diameter) dominated in regions with low chlorophyll concentrations (< 1 μg l⁻¹). About 30 % of the mean nanoplankton carbon consisted of heterotrophic flagellates. Heterotrophic microplankton carbon (> 20 μm diameter) was generally less significant. The variation in phytoplankton biomass was the result primarily of changes in cell density of pennate diatoms in the East Wind Drift, and of centric diatoms in the Weddell Sea and the Scotia Ridge region. Autotrophic and heterotrophic carbon as determined by microscopical analysis were compared with data for total particulate carbon, chlorophyll a, and adenosine triphosphate. Estimates for the C:chl ratio of autotrophs increased with decreasing concentrations of chlorophyll a, with mean values of 46 in bloom waters and 144 in 'blue water'. A C:ATP ratio for heterotrophic nanoplankton was estimated to be about 100, while that for heterotrophic microplankton may be lower. Algorithms, incorporating concentrations of chlorophyll a and ATP, are described which allow estimates of autotrophic and heterotrophic microbial biomass.

Our studies in the coastal waters in North Norway show that rates of photosynthesis of natural phytoplankton assemblages are strongly inhibited by solar UV radiation. When exposed to high irradiances of direct solar radiation, photosynthetic rates were increased by approximately 150% when all UV radiation was excluded from samples, with UVB radiation being responsible for approximately 50% of the total inhibition. There was no discernible threshold value for inhibition of photosynthesis by UV radiation, even at UV (280–400 nm) irradiances as low as 0.1 W m−2. When natural assemblages were incubated in situ, inhibition of photosynthetic rates were detectable down to 10 m, where solar irradiance was about 3% of the radiation incident on the sea surface. Based on the inhibition of photosynthetic rates at very low fluences of UV radiation, post-bloom assemblages of phytoplankton in North Norway and possibly also in the Arctic ocean appear to be more sensitive to solar UV radiation than phytoplankton from the Southern Ocean.

Recent studies have concluded that different water bodies in the ocean can contain different microbial communities. The goal of the present study was to determine if biogeographic patterns are present for aquatic microbes in waters which meet around the South Shetland Islands(SSI), Antarctica. Prokaryotic and eukaryotic marine microbial communities were monitored during the 2007 austral summer by use of polymerase chain reaction (PCR) and denaturing gradientgel electrophoresis (DGGE) of small subunit ribosomal DNA. Hydrographic properties, nutrients and chlorophyll a were also measured. There was an onshore to offshore gradient in temperature, salinity and iron concentration and a unimodal distribution of chlorophyll a concentration in rela-tion to the middle of this gradient that occurred near the SSI. The differences in microbial community structure among stations in the studied area were correlated with both geographical distance and environmental factors. For eukaryotes, the correlation was strongest for environment, where as it was strongest for geographical distance for the prokaryotes. Eukaryotic and prokaryotic community structures were highly correlated. Surface water from the Weddell Sea had a different community of eukaryotes than the water in the Antarctic Circumpolar Current in the Drake Passage, whereas the prokaryotic community was not significantly different. The area close to theSSI where the 2 water types mix had the highest chlorophyll concentration and significantly dif-ferent communities of eukaryotes and prokaryotes from both of the inflowing water types. These results suggest that the prokaryote community structure was more affected by productivity thanby environmental variables. KEY WORDS: Microbial biogeography. Microbial community. Natural iron enrichment. Southern Ocean.